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Image Unavailable-75841-148

POLYVIEW® Plus AP (Anti-Rabbit) Reagent, Enzo Life Sciences

Catalog Number: (75841-148)
Supplier: Enzo Life Sciences
Description: High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications.

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Image Unavailable-75968-950

Anti-PAX7 Mouse Monoclonal Antibody (CF488A) [clone: PAX7/497]

Supplier: Biotium
Description: The Pax gene family of nuclear transcription factors comprises nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Product Image-10800-520

Anti-TBC1D10C Rabbit Polyclonal Antibody

Catalog Number: (10800-520)
Supplier: Rockland Immunochemical
Description: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.
Product Image-97063-212

VWR® Stripping Buffers

Supplier: VWR
Description: VWR® offers three stripping buffers to accommodate the wide range of conditions that may be needed to remove different antibodies from blots.

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Image Unavailable-89358-214

Anti-Nphp1 Rabbit Polyclonal Antibody

Catalog Number: (89358-214)
Supplier: Genetex
Description: Juvenile nephronophthsis, an autosomal recessive cystic kidney disease, is the primary cause for chronic renal failure in children. The gene for nephronophthsis type 1 (NPHP1) has recently been identified. Its gene product, nephrocystin, is a novel protein of unknown function, which contains a src-homology 3 domain.NPHP1 expression has been studied in mouse and human systems. Expression in the human adult is widespread but relatively weak. In contrast, expression in adult mouse is strong and localized to the testis. In situ hybridization to whole mouse embryo demonstrates widespread and consistently strong expression throughout all developmental stages.It is hypothesized that the NPHP1 gene product, nephrocystin, is involved in signaling processes at focal adhesions (cell and extracellular matrix contact) and/or adherens junctions (cell-cell contact). The hypothesis is based on the fact that most src-homology 3 -containing proteins are part of focal adhesion signaling complexes. Additionally, nephrocystin binds to p130(cas), a prominent participant in focal adhesion signaling.
Product Image-97063-438

VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade

Supplier: VWR
Description: TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose

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Product Image-28150-288

Biodyne® Nylon Transfer Membranes, Cytiva (Formerly Pall Lab)

Supplier: Cytiva
Description: Biodyne® Transfer Membranes offer high sensitivity and low background for enhanced detection and resolution. They are ideal for nucleic acid detection.

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Product Image-10748-690

Anti-DISC1 Rabbit Polyclonal Antibody

Catalog Number: (10748-690)
Supplier: Prosci
Description: DISC1 Antibody: Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC1 has at least four known isoforms.
Product Image-10750-698

Anti-FRMPD1 Rabbit Polyclonal Antibody

Catalog Number: (10750-698)
Supplier: Prosci
Description: FRMPD1 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.
Product Image-10426-466

Anti-PATZ1 Rabbit Polyclonal Antibody

Catalog Number: (10426-466)
Supplier: Bioss
Description: The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]
Product Image-97062-122

VWR® TRIS-Borate-EDTA Buffer, 10X Liquid Concentrate, Ultra Pure Grade

Supplier: VWR
Description: TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose.A concentrated (10X), pH adjusted solution of Ultra Pure Grade Tris Buffer, ACS Grade Boric Acid & EDTA which when reconstituted to a working (1X) Solution concentration, contains 0.089M Tris, 0.089M Borate & 0.002M EDTA.

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Product Image-10801-438

Anti-TNFRSF12A Rabbit Polyclonal Antibody

Catalog Number: (10801-438)
Supplier: Rockland Immunochemical
Description: TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.
Product Image-10799-990

Anti-AMBRA1 Rabbit Polyclonal Antibody

Catalog Number: (10799-990)
Supplier: Rockland Immunochemical
Description: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
Product Image-10111-144

Anti-CHRNA7 Rabbit Polyclonal Antibody

Catalog Number: (10111-144)
Supplier: Prosci
Description: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA7 is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from CHRNA7 and a novel FAM7A gene.
Image Unavailable-102987-500

Biotinyl tyramide ≥97% (by HPLC)

Supplier: Adipogen
Description: Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents; improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.

Image Unavailable-10358-540

Anti-GFP Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))

Catalog Number: (10358-540)
Supplier: Bioss
Description: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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