Print…

You Searched For: Dithiothreitol+(DTT,+Cleland\'s+reagent)


26,977  results were found

Refine Results Sort by
SearchResultCount:"26977"

Sort Results

List View Easy View

Rate These Search Results

Product Image-75784-900

Essentials Spot Indole Reagent, Pro-Lab Diagnostics

Supplier: PRO-LAB DIAGNOSTICS INC CA
Description: Pro-Lab’s Spot Indole Reagent is to be used in the qualitative method to determine the ability of an organism to split indole from the tryptophan molecule.

SDS

Product Image-101643-330

Sample Buffers and Reagents, Protein Electrophoresis, Bio-Rad

Supplier: Bio-Rad
Description: Sample buffers are available for numerous applications, including native (nondenaturing) PAGE, SDS-PAGE, zymograms, and IEF

Certificates

Product Image-ST200436

AffinityScript cDNA Synthesis Kits, Agilent Technologies

Supplier: Agilent Technologies
Description: Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications.

Product Image-82022-660

2D Electrophoresis Reagents, G-Biosciences

Supplier: G-Biosciences
Description: G-Biosciences' FOCUS™ Protein Alkylation Kit contains an ultra pure, proteomic grade iodoacetamide reagent for blocking thiols by alkylation in order to prevent the reoxidation of proteins

Product Image-75927-870

AmpliScribe™ T7 High Yield Transcription Kit, Biotin-16-UTP, Biosearch Technologies

Catalog Number: (75927-870)
Supplier: Lucigen
Description: Easily synthesize high yields of RNA with this T7 RNA polymerase in vitro transcription kit.
Image Unavailable-77676-286

FlashTaq™ HotStart DNA Polymerase Kit

Supplier: EMPIRICAL BIOSCIENCE
Description: Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FlashTaq™ is completely suppressed prior to activation and regains full enzymatic activity in only 2 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in <i>E. coli</i>. FlashTaq™ HotStart DNA Polymerase is supplied in a storage buffer containing: HEPES, KCl, EDTA, DTT, Glycerol, and other stabilizers.

New Product

Product Image-103778-500

FastGene Scriptase II Reverse Transcriptase, Readymix, and cDNA kits, Bulldog Bio

Supplier: Bulldog Bio
Description: A reverse transcriptase engineered to deliver phenomenal results.

Product Image-76081-618

R&DNA™ T7 Polymerase, 'RNA Synthesis' with NTPs and dNTPs, Biosearch Technologies

Catalog Number: (76081-618)
Supplier: Lucigen
Description: Synthesize "RNA" transcripts that also contain both standard or modified dNTPs using this mutant T7 RNA polymerase
Product Image-75928-118

MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit, Biosearch Technologies

Catalog Number: (75928-118)
Supplier: Lucigen
Description: An optimized, complete system for the production of full-length cDNA from low amounts of total RNA.
Product Image-PAM1801

T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-75927-890

DuraScribe® T7 Transcription Kit, Synthesize RNase-resistant RNA In Vitro, Biosearch Technologies

Supplier: Lucigen
Description: Produce fully RNase A-resistant RNA that is perfectly suited for SELEX binding assays and RNA aptamer identification, as well as antisense RNA and RNA interference (RNAi) experiments.

Image Unavailable-10781-430

Lysobacter Recombinant Enzymogenes (from Hi-5 Insect Cells)

Supplier: Peprotech
Description: Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at, or adjacent to specific residues, or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes, including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Arg-C specifically cleaves at the carboxyl side of Arginine residues. Arg-C has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl-containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants, and by Co2+, Cu2+, Cd2+, and heavy metal ions. Recombinant Lysobacter Enzymogenes Arg-C is a 26.8 kDa protease consisting of 252 amino acid residues including a C-terminal His-Tag.

Product Image-PI21911

Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Invitrogen
Description: Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

SDS Certificates

Product Image-102963-634

Nuclear/Cytosolic Fractionation Kits, Cell Biolabs

Catalog Number: (102963-634)
Supplier: Cell Biolabs
Description: Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting
Image Unavailable-77983-463

TRC rac Dithiothreitol

Catalog Number: (77983-463)
Supplier: LGC Standards
Description: TRC rac Dithiothreitol

New Product


Image Unavailable-76177-312

Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Inquire for Price
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
49 - 64 of 26,977
no targeter for Bottom