You Searched For: Cloning+Kits+and+Ligation


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Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Supplier: New England Biolabs (NEB)
Description: This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. The pGGA destination plasmid (included), provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable <i>in vitro</i> transcription.

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Catalog Number: (97064-840)
Supplier: VWR
Description: VWR® Cyclo-Pure™ Agarose Gel Extraction Kit is a fast, spin column-based kit for the quick isolation of DNA fragments (40 bp - 100 kb) from agarose slices. In just 10 minutes, ultra-pure DNA can be extracted that is ready-to-use in restriction enzyme digestion, labeling, ligation, transformation, in vitro transcription and sequencing protocols. All reagents are room temperature stable.

Catalog Number: (76848-062)
Supplier: Oxford Nanopore Technologies
Description: Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.


Supplier: Cytiva
Description: illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.
Catalog Number: (PAC6711)
Supplier: Promega Corporation
Description: The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors. Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer.

Catalog Number: (76487-126)
Supplier: Oxford Nanopore Technologies
Description: PCR Primers (PRM) and PCR Adapters (PCA).


Supplier: Zymo Research
Description: The select-a-size DNA MagBead kit provides the fastest and easiest method to purify specific ranges of DNA fragments from PCR, endonuclease digestions, ligations, library preparations, adapter removal, etc.

Supplier: Oxford Nanopore Technologies
Description: MinION™ ― your personal, portable DNA and RNA sequencer.

Supplier: TELESIS BIO
Description: Generate Illumina-compatible, adapter-ligated, NGS libraries for whole genome sequencing in a simple automated workflow using the BioXp 3250.

Catalog Number: (10541-310)
Supplier: Abnova
Description: This Protein Phosphorylation Antibody Pair set comes with two Antibodies, one against the KIT protein, and the other against the specific Y721 phosphorylated site of KIT for use in Proximity Ligation Assay


Catalog Number: (10541-312)
Supplier: Abnova
Description: This Protein Phosphorylation Antibody Pair set comes with two Antibodies, one against the KIT protein, and the other against the specific Y721 phosphorylated site of KIT for use in Proximity Ligation Assay


Supplier: TELESIS BIO
Description: Generate Illumina-compatible, adapter-ligated, NGS libraries for whole genome sequencing in a simple automated workflow using the BioXp 9600.

Supplier: Zymo Research
Description: ZR Plasmid MiniPrep™ Classic is designed for efficient isolation of plasmid DNA from <i>E. coli</i> using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique fast-spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. Plasmid DNA purified using this kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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