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Supplier: KPL
Description: BCIP/NBT Membrane Phosphatase Substrate is ideal for immunoblotting and dot blot procedures

Catalog Number: (RLNBT-100)
Supplier: Rockland Immunochemical
Description: BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

Catalog Number: (97062-482)
Supplier: VWR
Description: Chromogenic substrate for the detection of alkaline phosphatase. Often used with INT or NBT to amplify the sensitivity of staining.

Catalog Number: (95059-176)
Supplier: KPL
Description: BCIP/NBT Membrane Phosphatase Substrate is ideal for immunoblotting and dot blot procedures

SDS


Catalog Number: (PAS3771)
Supplier: Promega Corporation
Description: For colorimetric detection of alkaline phosphatase activity.

Supplier: MP Biomedicals
Description: BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.

Catalog Number: (95059-338)
Supplier: KPL
Description: The Protein Detector BCIP/NBT Western Blot Kit is designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting

SDS


Catalog Number: (PI34070)
Supplier: Invitrogen
Description: The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

Supplier: Biotium
Description: Similar to BCIP/Na: the most widely used chromogenic phosphatase substrate, which forms a dark blue (max ~615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation.

Supplier: Adipogen
Description: NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase). Well-known scavenger of superoxide anions. Dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP). This substrate system produces an insoluble NBT diformazan end product that is blue in color and can be observed visually. When used with BCIP, it is suitable for detection of alkaline phosphatase in western blots, for immunohistological staining procedures and for colorimetric indication of bacterial infection in blood samples. Used as a redox indicator for enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels and pentose shunt dehydrogenses. The NBT/BCIP reaction is also used for colorimetric/spectrophotometric activity assays of oxidoreductases. One application is in activity stains in gel electrophoresis, such as with the mitochondrial electron transport chain complexes.

Supplier: Biotium
Description: is an oxidant widely used in conjuction with BCIP or BCIP derivatives to detect phosphotase activity or used as a substrate for dehydrogenase and other oxidases to assess cell viability, proliferation and cytotoxicity. NBT forms a dark blue-colored precipitate after enzymatic reduction.

Supplier: Surmodics
Description: BCIP/NBT Purple One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.

Catalog Number: (PI34035)
Supplier: Invitrogen
Description: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt

Supplier: Biotium
Description: is the most widely used chromogenic phosphatase substrate, which forms a dark blue (max ~615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation.

Catalog Number: (PI34042)
Supplier: Invitrogen
Description: The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

Supplier: MilliporeSigma
Description: NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50=3–4µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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