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Description: Amberlite® IRA-410 (Cl) ion-exchange resin

Catalog Number: TS202291000

Supplier: Thermo Scientific Chemicals

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Description: Styrene-DVB; Strongly basic anion exchange resin.

Catalog Number: AAA17734-36

Supplier: Thermo Scientific Chemicals

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Description: 1KG

Catalog Number: AAAL19567-30

Supplier: Thermo Scientific Chemicals

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Description: Amberlite® IRA-900 (Cl) ion-exchange resin

Catalog Number: TS202311000

Supplier: Thermo Scientific Chemicals

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Description: Amberlite® IRA-402 (Cl) ion-exchange resin

Catalog Number: TS369940010

Supplier: Thermo Scientific Chemicals

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Description: Chloride form quaternary ammonium functionality.Strongly basic, macroreticular resin of moderately high porosity with benzyltrialkylammonium functionality.

Catalog Number: IC15031990

Supplier: MP Biomedicals

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Catalog Number: AAB25556-22

Supplier: Thermo Scientific Chemicals

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Description: Amberlite|r IRA-96, ion exchange resin

Catalog Number: AAH32475-0B

Supplier: Thermo Scientific Chemicals

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Description: Acrylic-DVB; Weakly basic gel type resin

Catalog Number: AA42253-A1

Supplier: Thermo Scientific Chemicals

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Description: Styrene-DVB; Strongly acidic macroreticular cation exchange resin

Catalog Number: AA42252-A1

Supplier: Thermo Scientific Chemicals

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Description: Amberlite® HPR4200 (Cl) ion-exchange resin

Catalog Number: TS475860025

Supplier: Thermo Scientific Chemicals

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Description: Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Catalog Number: IC821527

Supplier: MP Biomedicals

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Description: CAS: 527-20-8; EC No: 208-410-3; MDL No: MFCD00014769; RTECS: BY7910000 UN No: UN2811; Haz Class: 6.1; Packing Group: III Solid; Linear Formula: C6Cl5NH2; MW: 265.35 Melting Point: 232-234°; Flash point: <gt/>100°C (<gt/>212°F) Air Sensitive, Light Sensitive

Catalog Number: BT134950-5G

Supplier: BeanTown Chemical

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Description: For olefin isomerization, preparation of acetals and hemiacetal esters.
Soluble in acetonitrile, acetone, chloroform.

Catalog Number: AA10002-03

Supplier: Thermo Scientific Chemicals

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Description: Amberlite® 200, powder 200

Catalog Number: IC15027891

Supplier: MP Biomedicals

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Description: Amberlite® IR-120 (H) moisture 53-58%

Catalog Number: 77877-901

Supplier: Ambeed

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