You Searched For: Amberlite®+HPR900+(OH)

Catalog Number: (ALA118726-500G)

Supplier: ALADDIN SCIENTIFIC

Description: Strong acid cation exchange resin (Na)

New Product

Supplier: MP Biomedicals

Description: Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

SDS Certificates

Catalog Number: (BDH9248-500G)

Supplier: VWR International

Description: White solid.

SDS Certificates

Catalog Number: (BDH24388.320)

Supplier: VWR International

Description: Glycerine ≥99.5%, AnalaR NORMAPUR® ACS, redistilled, VWR Chemicals BDH®

SDS Certificates

Supplier: VWR International

Description: Clear liquid. Suitable for organic synthesis, cleaning, and prep scale synthesis.

SDS Certificates

Supplier: VWR International

Description: (±)-1,2-Propanediol ≥99.5% USP, Multi-Compendial

Certificates

Supplier: VWR International

Description: Clear liquid. Suitable for histology and cytology applications. Soluble in water.

SDS Certificates

Supplier: VWR International

Description: Ethanol denatured ACS (denatured with 5% isopropanol and 5% methanol), VWR Chemicals BDH®

SDS Certificates

Catalog Number: (BDH1160-4LP)

Supplier: VWR International

Description: Clear, colorless liquid. Denatured with 3.8–4.5% methanol and 4.0–5.0% 2-propanol. Suitable for cytology and histology.

SDS Certificates

Supplier: VWR International

Description: Meets ACS specifications for general use.

SDS Certificates

Catalog Number: (BDH2029-1GLP)

Supplier: BDH

Description: Methanol ≥99.9%, Semi Grade for the electronics industry, VWR Chemicals BDH®

SDS Certificates

Supplier: VWR International

Description: Clear liquid. Soluble in water. Meets ACS specifications.

SDS Certificates

Catalog Number: (95041-464)

Supplier: VWR International

Description: Methyl Alcohol, anhydrous

SDS Certificates

Catalog Number: (BDH67002.400)

Supplier: VWR International

Description: Ethanol 95-97% (v/v) (denatured with 5% methanol), BDH HiPerSolv CHROMANORM for HPLC (denatured with 5% isopropanol and 5% methanol), VWR Chemicals BDH®

SDS Certificates

Supplier: VWR International

Description: Clear, colorless liquid.

SDS Certificates

Catalog Number: (BDH83635.400)

Supplier: VWR International

Description: 1-Propanol ≥99.5%, HiPerSolv CHROMANORM® for HPLC, VWR Chemicals BDH®

SDS Certificates