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Anti-His-tag Mouse Monoclonal Antibody [clone: 6-His]

Catalog Number: (76201-852)

Supplier: Enzo Life Sciences

Description: Recommended Applications: IF, IP, WB

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Mouse Recombinant IL1B (from E. coli)

Catalog Number: (75794-046)

Supplier: Prosci

Description: IL-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation and fibroblast growth factor activity. IL-1beta belongs to the IL-1 family of proteins that are involved in the inflammatory response. IL-1beta in most situations requires the inflammasome complex to be cleaved and secreted.

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Anti-TLR9 Rat Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: M9.D6]

Catalog Number: (75843-286)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The M9.D6 monoclonal antibody specifically reacts with mouse CD289 also known as toll-like receptor 9 (TLR9). CD289 is involved in the activation of innate immunity, in acquired immune responses, and autoimmune diseases. It is involved in the immune systemâ€™s response to unmethylated CpG dinucleotide sequences such as those found in bacterial, viral, or synthetic DNA. CD289 assists in pathogen recognition and is expressed by subtypes of dendritic cells and B cells.

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Pierce™ High pH Reversed-Phase Peptide Fractionation Kit, Thermo Scientific

Catalog Number: (PI84868)

Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

SDS Certificates

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Human Recombinant IGFL3 (from HEK293 cells)

Catalog Number: (75794-054)

Supplier: Prosci

Description: Insulin-growth factor-like gene family is a new family of proteins consisting of four proteins in humans (IGFL1 to 4) and one in mice (mIGFL). mIGFL is expressed in normal skin in mice and further upregulated during inflammation responses in skin or after skin wounding. In human only IGFL1 expression is increased in psoriatic skin samples. mIGFL and human IGFL1 and 3 interact with specificity and high affinity to a novel receptor named IGF-like family receptor 1 (formerly TMEM-149). Analysis of the amino acid sequence of IGFLR1 indicated that this receptor is likely a novel member of the TNF-R family. IGFLR1 is expressed most abundantly on mouse T cells, suggesting that mIGFL and IGFL1 produced in the skin may potentially exert regulatory functions on T cell responses.

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Anti-Cop9 Signalosome Csn6 Subunit Rabbit Polyclonal Antibody

Supplier: Enzo Life Sciences

Description: The polyclonal antibody to Csn6 was generated by immunisation of rabbits with a synthetic peptide corresponding to residues [283-297] of the human Csn6 sequence. The antibody has been characterised by one-dimensional Western blotting. Vial contains a partially purified immunoglobulin preparation suspended phosphate-buffered saline containing 0.01M sodium azide. The COP9 signalosome, once defined as a repressor complex of light activated development in Arabidopsis, has recently been found in humans and is probably present in most multicellular organisms. The COP9 signalosome is closely related to the lid sub-complex of the 26S proteasome in structural composition and probably shares a common evolutionary ancestor. A multifaceted role of the COP9 signalosome in cell-signalling processes is hinted at by its associated novel kinase activity, as well as the involvement of its subunits in regulating multiple cell-signalling pathways and cell-cycle progression. The molecular genetic studies in Arabidopsis suggest that the complex functions as part of a highly conserved regulatory network, whose physiological rüle in animals has yet to be determined. The COP9 signalosome, also known as the the COP9 complex and JAB1-containing signalosome, is a conserved nuclear protein complex found in plants and animals. It is composed of eight distinct subunits, designated S1 to S8 (very recently re-designated Csn1-Csn8) according to the molecular weight of the mammalian complex subunits. Subunit composition and the subunit sequences are substantially conserved between the mammalian and the plant complexes, implying that the complex has a conserved cellular function in higher eukaryotic organisms. The role and function of Csn6 has yet to be determined although this subunit is known to interact with subunits Csn2 and Csn7 within the COP9 signalosome.

Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)

Supplier: Adipogen

Description: Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.

Anti-CTLA4 Armenian Hamster Monoclonal Antibody (PE (Phycoerythrin)) [clone: UC10-4F10-11]

Catalog Number: (75842-244)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The UC10-4F10-11 monoclonal antibody specifically reacts with the mouse Cytotoxic T-Lymphocyte Antigen-4 (CTLA-4), also known as CD152. It is a protein with a structure similar to CD28 regarding the genomic organization, amino acid sequence, and structure. CTLA-4 is expressed on activated T cells and CD25+/CD4+ Treg lymphocytes and binds the members of the B7 family, B7-1 (CD80) and B7-2 (CD86), with higher affinity than CD28. CD28 seems to provide opposing signal to T lymphocytes, while CD152 inhibits the T lymphocytes progression to an activated state and their proliferation, CD28 is a costimulator. The mouse UC10 -4F10-11 monoclonal antibody does not cross-react with the rat leukocytes.

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Human Recombinant CD137 (from HEK293 cells)

Catalog Number: (75794-010)

Supplier: Prosci

Description: CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor family. CD137 is expressed by activated T cells, dendritic cells, NK cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. CD137 was shown to enhance immune activity to eliminate tumors in mice.

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Anti-CTLA4 Armenian Hamster Monoclonal Antibody (APC (Allophycocyanin)) [clone: UC10-4F10-11]

Catalog Number: (75842-250)

Supplier: BIOGEMS INTERNATIONAL INC.

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Anti-FAM123A Rabbit Polyclonal Antibody

Catalog Number: (103285-170)

Supplier: Novus Biologicals

Description: The FAM123A Antibody from Novus Biologicals is a rabbit polyclonal antibody to FAM123A. This antibody reacts with human. The FAM123A Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.