XIT™ DNA for Protein-Free DNA Isolation, G-Biosciences
Supplier: G-Biosciences
G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences' XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation, and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, Buccal cells, plant tissues, mouse tail, yeast, and FFPE tissue. G-Biosciences' XIT™ DNA kit procedures remove contaminants and enzyme inhibitors, allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences' XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio, between 1.7 and 1.9 (with the exception of the Buccal cells kit with a ratio between 1.8 and 2.0), and are up to 200 kb in size.
G-Biosciences' XIT™ Genomic DNA kits for Animal/Plant/FFPE Tissues, Mouse Tail, and Buccal Cells all use a three main step procedure that includes: rapid and efficient cell lysis then enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Tissue kit is designed for the isolation of genomic DNA from fresh, frozen or methanol/acetone fixed tissues. The kit is supplied with three protocols for the isolation of: 1 - 10 mg fresh or frozen tissue, 50 - 100 mg fresh or frozen tissue or 5 - 10 mg fixed tissue.
G-Biosciences' XIT™ Genomic DNA from Plant Tissue kit is designed for the isolation of genomic DNA from fresh or frozen plant tissues.
G-Biosciences' XIT™ Genomic DNA from FFPE Tissue kit is designed for the isolation of genomic DNA from formalin fixed, paraffin embedded tissue.
G-Biosciences' XIT™ Genomic DNA from Mouse Tail kit is designed for the isolation of genomic DNA from 5 mm sections of mouse tail.
G-Biosciences' XIT™ Genomic DNA from Blood kit is designed for the isolation of genomic DNA from blood, bone marrow and buffy coat. The kit uses four main steps that are red blood cell lysis, rapid & efficient nuclear lysis, complete protein precipitation followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Cells kit is designed for the isolation of genomic DNA from adherent or suspension cultured cells. The kit uses rapid & efficient nuclear lysis and complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Gram Negative Bacteria kit is designed for the isolation of genomic DNA from overnight cultures. XIT™ Genomic DNA from Gram Negative Bacteria kit protocol is designed to use 1 ml overnight culture; however the protocol can be easily adapted for larger tissue sample sizes. The kit uses three main steps that are rapid & efficient bacterial lysis, complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Gram Positive Bacteria kit is designed for the isolation of genomic DNA from overnight cultures. The kit uses three main steps that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Yeast kit is designed for the isolation of genomic DNA from overnight yeast cultures. The kit uses three main steps that are rapid and efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation. XIT™ Genomic DNA from Yeast kit protocol is designed to use 1 ml overnight culture; however, the protocol can be easily adapted for larger tissue sample sizes.
G-Biosciences' XIT™ Mitochondrial DNA kit combines G-Biosciences FOCUS™ Mitochondria technology and XIT™ DNA isolation technology in a single kit. The kit uses subcell buffers to lyse cells and animal tissues and remove cellular proteins and nuceli from a highly enriched, intact and active mitochondrial fraction. The mitochondria are then lyzed with the included lysis buffer. The lysate is treated with G-Biosciences' LongLife™ Proteinase K to degrade the proteins, which are then precipitated and the mitochondrial DNA is isolated with alcohol precipitation. The kit protocol also contains protocols for isolation of mitochondra from soft (brain and liver) and hard (cardiac and skeletal muscle) tissue.
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