Peptide Coupling Kits, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' HOOK™ Peptide Coupling (Amine Reactive) Kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins
This kit uses the chemical heterobifunctional crosslinker EDC to couple peptides to carrier proteins. EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein.
G-Biosciences' HOOK™ Peptide Coupling (Sulfhydryl Reactive) Kit is designed for the coupling of peptides to carrier proteins, utilizing a sulfhydryl group in the peptide. This kit exploits the chemical cross-linker sulfoSMCC to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins.
The N-hydroxysuccinimide (NHS) ester in sulfoSMCC reacts with primary amines to form covalent amide bonds and the maleimide group reacts with sulfhydryl groups to form stable thioether bonds. If peptides do not contain a sulfhydryl group then G-Biosciences recommends and supplies Traut’s reagent (2-IminothiolaneHCl). Traut’s reagent modifies primary amines, located at the N-terminus and on lysine side chains, introducing a sulfhydryl group that is fully compatible with this kit.
G-Biosciences' HOOK™ Peptide Coupling Kits (both Amine and Sulfhydryl Reactive) utilize G-Biosciences' Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands on time and effort.
Información para pedidos: Accessories available.
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