ProteaseArrest™ Protease Inhibitor Cocktails, G-Biosciences
Supplier: G-Biosciences
ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution
ProteaseArrest™ Outperforms Tablet Cocktails: The ProteaseArrest™ format allows delivery of optimized concentrations of protease inhibitor, for example 2X or higher concentrations can be added for tissues with higher than normal protease concentrations; a feature not possible with tablet format protease inhibitor cocktails. In our study, a 1X concentration of ProteaseArrest™ inhibits over 95% of protease activities (e.g. 0.5mg/ml Mouse Pancreas Extract). The ProteaseArrest™ protease inhibitor cocktails demonstrate greater inhibition levels compared to similar protease inhibitor cocktails, including tablet formats. In independent studies, researchers have found that ProteaseArrest™ outperforms several leading manufacturer's protease inhibitor cocktails, including tablet formats, in the purification of plant proteins.
ProteaseArrest™ shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during preparation of samples and protein purification from animal tissues, plants, yeast, bacteria, and fungal lysates. For enhanced inhibition, ProteaseArrest™ is supplied in species specific formats for plant, bacteria, mammalian, yeast, fungal and a specialized recombinant protein purification inhibitor. These cocktails are based on ProteaseArrest™ but are enhanced with specialized inhibitors. ProteaseArrest™ is also available as single use aliquots that are suitable for >95% protease inhibition in 10ml solutions. ProteaseArrest™ is also offered as a dry format, called ProteCEASE™, for those who prefer reconstitution prior to use. For protection of proteins from proteolysis in such applications as 2D electrophoresis, we offer FOCUS™ ProteaseArrest™.
It is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µL ProteaseArrest™ per mL sample. For samples with higher than normal protease levels, the volume of ProteaseArrest™ added can be increased for greater inhibition levels. Protease levels can easily be assayed with our Protease Assays. The cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases. An optional EDTA solution is provided for enhanced metalloprotease inhibition. It is not present in the actual ProteaseArrest™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC).
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