ProteaseArrest™ Protease Inhibitor Cocktails, G-Biosciences

Supplier: G-Biosciences

786-333 786-108 786-326T 786-327 786-328 786-336 786-331 786-108F 786-437 786-334 786-332 786-329 786-433 786-330 786-326 786-335 786-376
78000-040EA 126.3 USD
78000-040 82022-478 95029-202 78000-024 78000-026 78000-032 78000-036 82021-532 71003-168 78000-028 78000-038 95043-418 71003-160 78000-034 78000-022 78000-030 95057-582
ProteaseArrest™ Protease Inhibitor Cocktails, G-Biosciences
Cell Culture Supplements and Additives

ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution


ProteaseArrest™ Outperforms Tablet Cocktails: The ProteaseArrest™ format allows delivery of optimized concentrations of protease inhibitor, for example 2X or higher concentrations can be added for tissues with higher than normal protease concentrations; a feature not possible with tablet format protease inhibitor cocktails. In our study, a 1X concentration of ProteaseArrest™ inhibits over 95% of protease activities (e.g. 0.5mg/ml Mouse Pancreas Extract). The ProteaseArrest™ protease inhibitor cocktails demonstrate greater inhibition levels compared to similar protease inhibitor cocktails, including tablet formats. In independent studies, researchers have found that ProteaseArrest™ outperforms several leading manufacturer's protease inhibitor cocktails, including tablet formats, in the purification of plant proteins.


ProteaseArrest™ shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during preparation of samples and protein purification from animal tissues, plants, yeast, bacteria, and fungal lysates. For enhanced inhibition, ProteaseArrest™ is supplied in species specific formats for plant, bacteria, mammalian, yeast, fungal and a specialized recombinant protein purification inhibitor. These cocktails are based on ProteaseArrest™ but are enhanced with specialized inhibitors. ProteaseArrest™ is also available as single use aliquots that are suitable for >95% protease inhibition in 10ml solutions. ProteaseArrest™ is also offered as a dry format, called ProteCEASE™, for those who prefer reconstitution prior to use. For protection of proteins from proteolysis in such applications as 2D electrophoresis, we offer FOCUS™ ProteaseArrest™.


It is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µL ProteaseArrest™ per mL sample. For samples with higher than normal protease levels, the volume of ProteaseArrest™ added can be increased for greater inhibition levels. Protease levels can easily be assayed with our Protease Assays. The cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases. An optional EDTA solution is provided for enhanced metalloprotease inhibition. It is not present in the actual ProteaseArrest™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC).

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