4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) alkaline phosphatase substrate, Sigma-Aldrich®

Synonyms: 4-Nitrophenyl phosphate disodium salt hexahydrate, Phosphatase substrate, p-Nitrophenyl phosphate disodium salt hexahydrate, Phosphoric acid 4-nitrophenyl ester disodium salt hexahydrate, 4-Nitrophenyl phosphate disodium hexahydrate, pNPP disodium salt hexahydrate, di-Sodium 4-nitrophenyl phosphate hexahydrate

P4744-50G P4744-5G P4744-10G P4744-25G P4744-1G
220203-891EA 4169.23 USD
220203-891 220203-892 220203-888 220203-890 220203-889
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) alkaline phosphatase substrate, Sigma-Aldrich®
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)

Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP). Substrate for the determination of acid and alkaline phosphatases.


Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Formula: C₆H₄NNa₂O₆P·6H₂O
MW: 371.144 g/mol
Melting Pt: 300 °C
Storage Temperature: Freezer
MDL Number: MFCD00066288
CAS Number: 333338-18-4
EINECS: 224-246-5

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR