3,3',5,5'-Tetramethylbenzidine (TMB) ≥99%, Sigma-Aldrich®

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

860336-1G 860336-5G 860336-100MG 860336-25G

220098-047EA 54.28 USD

220098-047 220098-049 220098-046 220098-048

3,3',5,5'-Tetramethylbenzidine (TMB) ≥99%, Sigma-Aldrich®

3,3',5,5'-Tetramethylbenzidine (TMB)

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures.

The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Formula: C₁₆H₂₀N₂
MW: 240.35 g/mol
Boiling Pt: 402 °C (1013 hPa)
Melting Pt: 166…170 °C
Storage Temperature: Ambient

MDL Number: MFCD00007748
CAS Number: 54827-17-7
EINECS: 259-364-6

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