HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid, high purity

Supplier: VWR

Synonyms: HEPES free acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid

0511-1KG 0511-250G 0511-50G
97061-822CS 2505.52 USD
97061-822 97061-824 97061-826
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid, high purity
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid)

One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.


Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Melting Pt: 234 °C
Density: 1.448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
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Specification Test Results

DNase none detected
Heavy Metals (as Pb) <= 0.0005 %
Iron <= 0.0005 %
pKa @20°C 7.45 - 7.65
Protease none detected
Purity (Dry Basis) > 99 %
Residue on Ignition < 0.2 %
RNase none detected

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