Pierce™ LightShift® Chemiluminescent EMSA Kit, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

20148 20148X
PI20148EA 805.38 USD
PI20148 PI20148X
Pierce™ LightShift® Chemiluminescent EMSA Kit, Thermo Scientific
Electrophoresis Reagents Western Blotting Reagents

LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.


  • Excellent for detecting low-abundance proteins in nuclear extracts
  • Sensitivity that surpasses radioactive and digoxigenin methods
  • Compatible with previously established binding conditions for popular DNA-protein interactions
  • Includes EBNA Optimization and Control Kit to help new users develop a working assay and understand the methods used to confirm binding interaction specificity


Información para pedidos: The principle for LightShift EMSA Detection is similar to a Western blot. One technique that is central to studying gene regulation and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA).

The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide or agarose gel electrophoresis. Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay. DNA targets can be synthesized with 5' or 3' biotin labels or they can be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Product No. 89818). Nuclear, cytosolic or whole cell protein extracts can be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit.


Información de suministro: Kit includes LightShift™ EMSA Optimization and Control Kit and Chemiluminescent Nucleic Acid Detection Module.

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