Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, white crystalline powder, Ultrapure
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris), Tromethamine, Tris(hydroxymethyl)methylamine, 2-Amino-2-(hydroxymethyl)propane-1,3-diol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trizma(R) base, TRIS base, THAM
Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic; but, if needed, it can be dried at 100°C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.
- Ultra Pure Grade
- White Crystalline Powder
- pKa at 25°C= 8.1
- Tris solutions can be autoclaved (121°C, 15 psi, 15 minutes) or sterile filtered.
- Room Temperature, desiccate
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions (Nathalie Rouleau,(2008),Highly Sensitive Assays for Sumoylation and SUMO-Dependent Protein-Protein Interactions,Analytical Biochemistry,375(2),pages: 364-366).
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Formula:
NH₂C(CH₂OH)₃ MW: 121.14 g/mol Boiling Pt: 219…220 °C (10 mmHg) Melting Pt: 165…175 °C Density: 1.328 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00004679 CAS Number: 77-86-1 EINECS: 201-064-4 Merck Index: 13,09842 |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥99.9% |
| FTIR | Conforms to Standard |
| pH (5% aq soln) | 10-11.5 |
| OD260 nm (5% aq soln) | ≤0.1 |
| Loss on Drying | ≤1% |
| Solubility (40% aq soln) | Clear, Colorless Solution |
| Residue on Ignition | ≤0.1% |
| Heavy Metals (as Pb) | ≤5 ppm |
| Iron (Fe) | ≤5 ppm |
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