NEXTFLEX™ RiboNaut rRNA (HMR) Depletion Kits
Supplier: REVVITY HEALTH SCIENCES, INC.
The NEXTFLEX® RiboNaut™ rRNA depletion kit (HMR) effectively removes rRNAs from mammalian total RNA while enabling labs to find additional RNA species in a sample, not only limited to intact mRNAs.
- Hybridization and bead-based protocol for rRNA depletion
- Optimized for use with 5 ng to 1 µg of total RNA as starting material
- Versions are available for automated and manual rRNA depletion
The NEXTFLEX® RiboNaut™ rRNA depletion kit (Human/mouse/rat) is designed to be compatible with human, mouse, and rat total RNA. The method used in the NEXTFLEX® RiboNaut™ rRNA depletion kit is based on subtractive hybridization, using a mixture of biotinylated oligonucleotides complementary to rRNAs.
After hybridization of the bait oligos to total RNA, the rRNA/bait complex and excess bait oligos are removed by capture onto streptavidin-coated magnetic beads. The remaining RNA, which has been depleted of both cytoplasmic and mitochondrial rRNAs, is then purified and concentrated using magnetic beads.
Certificaciones: Manufactured in ISO 9001 Facility.
Precaución: For research use only. Not for use in diagnostic procedures.
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