Sulfo-DIBMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Usage: Membrane protein solubilization & stabilization
Full-Name: Poly(diisobutylene-alt-N´,N´-/dimethyl(maleimidopropyl))ammonium propane sulfonate copolymer, sodium salt in 50 mM HEPES, pH 7.5
Formula Weight: 8,000 g/mol ± 1000 g/mol
Buffer: 50 mM HEPES
pH upon addition of dH2O: 7.5 ± 0.3
Total pH stability: 4-10
State: Lyophilized Powder
Recommended concentration range upon use: 1% to 5%
Solubility: > 10 % in H2O
Absorbance at 280 nm: < 0.3 (1 % solution)
Mg2+ Tolerance: over 100 mM
Ca2+ Tolerance: over 100 mM
Color: White to slightly yellow
Precaución: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...