Glyco-DIBMA in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.
State: Lyophilized Powder, to be solved with water;
Molecular weight: 15701 g/Mol;
pH after solving: 7.5;
Solubility: >10%;
Divalent cationic tolerance: < 4 mM;
Adsorbance (280 nm, 1% solution): > 0.3;
Buffer: HEPES;
Specific gravity: 1.1
Precaución: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.
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