PureCube HiCap StrepTactin® Agarose XL
Supplier: CUBE BIOTECH
57303
57305
57310
77393-326EA
1265.8
USD
77393-326
77393-328
77393-330
PureCube HiCap StrepTactin® Agarose XL
Protein Purification
Protein Purification Reagents
Our PureCube HiCap StrepTactin® agarose XL resin beads consist of 6% cross-linked agarose coupled to the StrepTactin protein.
- Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
- Specificity: Affinity to Strep-tagged (WSHPQFEK) proteins
- Binding capacity: >5 mg/ml
- Bead Ligand: StrepTactin®
- Bead size: 400 μm
- Matrix: 6% cross-linked agarose
- Filling quantity: Delivered as a 50% suspension
- Chemical stability: 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-glucopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sarcosine, 0, 1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
- Short-term storage: In neutral buffer at 4 °C
- Long-term storage: In neutral buffer with sodium azide at 4 °C
- Required equipment:
- Lysis Buffer
- Wash Buffer
- Elution Buffer (incl. Rho1D4 peptide)
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and Micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
The PureCube HiCap StrepTactin® agarose XL resin beads enable protein yields of up to 5 mg protein per ml resin and were developed for FPLC and batch spin-based protein purification.
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