Virus Recombinant MERS-COV Trim Spike Protein (from Mammalian Cells)
Supplier: ReVacc Scientific
A recombinant trimeric form of the spike glycoprotein (S protein) of MERS-Cov (GenBank: JX869059.2) was produced by human embryonic kidney HEK293F cells, followed by purification.
- Protein function: Bind to its receptor
This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (V1060P and L1061P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling.
This product has been run on SDS-PAGE with reduced condition and Native blue gels, with results proving trimerization and glycosylation. The binding to one MERS control mAb and published MERS27 antibody was tested in ELISAs.
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