Virus Recombinant SARS-COV-2 Trim Spike D614G Mutant 100 µg (from Mammalian Cells)

Supplier: ReVacc Scientific

J23V36-100UG J23V36-500UG
76515-424EA 758.86 USD
76515-424 76515-426
Virus Recombinant SARS-COV-2 Trim Spike D614G Mutant 100 µg (from Mammalian Cells)
Proteins and Peptides

A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (GenBank: MN908947) bearing a mutated S protein from D (Asp) to G (Gly) at site of 614 was produced by human embryonic kidney HEK293F cells, followed by purification.


  • Protein function: Bind to its receptor


This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling.


This product has been run on SDS-PAGE with reduced condition and Native blue gels as well as SEC, with results proving trimerization and glycosylation. The binding to two control mAbs and CR3022 was tested in ELISAs.

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SPECIFICATIONS

Protein/Peptide Name SARS-COV-2 trim spike D614G mutant
Environmentally Preferable
Protein Synonyms SARS-COV-2 trimeric spike protein-D614G mutant (His and Strep Tags)
Protein/Peptide Type Recombinant
Species Virus
Source Mammalian cells
Conjugation Unconjugated
Gene ID MN908947
Protease-free N
Animal-Free Y
Nuclease-free N
Carrier-Free Y
Endotoxin-free N
Endotoxin Level None detected
Purity 90 - 100%
Concentration 0.2 mg/ml
Formulation Frozen liquid. In PBS pH 7.5
Molecular Weight ~ 140 kDa
Storage Conditions –80 °C
Shipping Temperature 70
Tested Applications ELISA, SDS-PAGE, NativeBlue, SEC

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