Virus Recombinant SARS-COV-2 Trim Spike Wuhan Strain 100 µg (from Mammalian Cells)

Supplier: ReVacc Scientific

Cartoon illustration of protein

Purity test by SEC

Purity test by gel

Functional binding test by ELISA assay

Cartoon illustration of protein

Purity test by SEC

Purity test by gel

Functional binding test by ELISA assay

C40N10-100UG C40N10-500UG

76515-420EA 758.86 USD

76515-420 76515-422

Virus Recombinant SARS-COV-2 Trim Spike Wuhan Strain 100 µg (from Mammalian Cells)

Proteins and Peptides

A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (Wuhan strain, GenBank: MN908947) was produced by human embryonic kidney HEK293F cells, followed by purification.

Protein function: Bind to its receptor

This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling.

This product has been run on SDS-PAGE with reduced condition and Native blue gels as well as SEC, with results proving trimerization and glycosylation. The binding to two control mAbs and CR3022 was tested in ELISAs.

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