NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content.
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
- Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
- Generate high yields with increased reaction efficiencies and minimized sample loss
- Includes SPRIselect beads
The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification, and the gold standard SPRIselect size selection and clean-up beads. Please note that adaptors and primers are not included in the kit and are available separately.
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