NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads, New England Biolabs

Supplier: New England Biolabs (NEB)

E6177L E6177S
103529-164EA 2813 USD
103529-164 103529-166
NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads, New England Biolabs
Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content.


  • Perform fragmentation, end repair and dA-tailing with a single enzyme mix
  • Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
  • Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
  • Generate high yields with increased reaction efficiencies and minimized sample loss
  • Includes SPRIselect beads


The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification, and the gold standard SPRIselect size selection and clean-up beads. Please note that adaptors and primers are not included in the kit and are available separately.

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