NEBExpress MBP Fusion and Purification System, New England Biolabs

Supplier: New England Biolabs
E8201S
76423-408EA 800 USD
76423-408
NEBExpress MBP Fusion and Purification System, New England Biolabs
Protein Purification Protein Purification Kits
The NEBExpress MBP fusion and purification system takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation
  • Gentle elution with maltose; no detergents or harsh denaturants required
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