Exo-CIP Rapid PCR Cleanup Kits, New England Biolabs
Supplier: New England Biolabs (NEB)
The Exo-CIP Rapid PCR cleanup kit contains optimized formulations of thermolabile Exonuclease I and thermolabile Calf Intestinal Phosphatase (CIP) and is used to rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification.
- Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
- Reaction complete in 4 minutes
- Thermolabile formulation can be heat inactivated in 1 minute at 80 °C
- PCR product can be used directly in downstream applications
Degradation occurs in only 4 minutes at 37 °C, and is immediately followed by rapid inactivation of the enzymes by heating for 1 minute at 80 °C. In just 5 minutes, the PCR product is ready for downstream analysis, such as Sanger Sequencing, SNP detection, or library preparation for NGS. The Exo-CIP Rapid PCR cleanup kit is compatible with all commonly-used PCR buffers.
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