Monarch® HMW DNA Extraction Kits for Cells and Blood, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® HMW DNA extraction kit for cells and blood provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from cultured cells and whole blood.
- Novel glass bead based workflow is fast and user-friendly (cells: 30 min, blood: 60 min)
- Isolate DNA into the Mb size range from cells and blood samples
- Easily tune the size of isolated DNA by adjusting the agitation speed during lysis
- Obtain excellent yields of highly-pure and intact DNA; ideal for long read sequencing
- RNase A and Proteinase K are included
Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50 to 250 kb for the standard protocol and into the Mb range when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For cells, the process time is only 30 min, while blood samples require erythrocyte lysis and are processed in 60 min. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing.
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