Human Recombinant FGL1 (from HEK293 cells)

Supplier: ACROBIOSYSTEMS INC MS

FG1H5258100UG FG1H52581MG
103790-364EA 387.69 USD
103790-364 103790-366
Human Recombinant FGL1 (from HEK293 cells)
Proteins and Peptides

Human FGL1 Protein, Fc Tag, ACROBiosystems

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SPECIFICATIONS

Protein/Peptide Name FGL1
Environmentally Preferable
Protein Synonyms Hepassocin|HP-041|HFREP1|FGL1|LFIRE-1|HFREP-1
Protein/Peptide Type Recombinant
Species Human
Source HEK293 cells
Conjugation Unconjugated
Gene ID Q08830-1
Endotoxin Level Low
Endotoxin Content Less than 1.0 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag (Cat. No. FG1-H5258) with a linear range of 0.02-0.156 μg/mL (QC tested). Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, Fc Tag (Cat. No. FG1-H5258): Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.42303 μg/mL (Routinely tested). Loaded Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) on AMC Biosensor, can bind Human FGL1, Fc Tag (Cat. No.FG1-H5258) with an affinity constant of 43.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS assay shows that Human FGL1, Fc Tag (Cat. No. FG1-H5258) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 3 μg/mL (Routinely tested).
Purity >95% as determined by SDS-PAGE.
Formulation Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Molecular Weight 60.5 kDa
Storage Conditions –20 °C
Shipping Temperature RT
Tag sequence Fc Tag

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