5-Bromo-4-chloro-3-indolyl-α-D-galactoside

Supplier: RPI

Synonyms: X-a-Gal, 5-Bromo-4-Chloro-3-Indolyl-a-D Galactopyranoside, X-alpha-Gal

B71900-0.25 B71900-1.0
76345-234EA 320.3 USD
76345-234 76345-236
5-Bromo-4-chloro-3-indolyl-α-D-galactoside
5-Bromo-4-chloro-3-indolyl-alpha-D-galactoside

Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae.


Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay.


Precaución: Caution: Research or further manufacturing use only, not for food or drug use.

Formula: C₁₄H₁₅BrClNO₆
MW: 408.63 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00063780
CAS Number: 107021-38-5

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Specification Test Results

Appearance: Colorless or white to off- white crystalline powder
Solution Test (1% in Methanol): Clear and colorless
Turbidity (1% in Methanol): ≤ 4.0 NTU
Water (KF): ≤ 1.0 %

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