5-Bromo-4-chloro-3-indolyl-α-D-galactoside
Supplier: RPI
Synonyms:
X-a-Gal, 5-Bromo-4-Chloro-3-Indolyl-a-D Galactopyranoside, X-alpha-Gal
Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae.
Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay.
Precaución: Caution: Research or further manufacturing use only, not for food or drug use.
Formula:
C₁₄H₁₅BrClNO₆ MW: 408.63 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00063780 CAS Number: 107021-38-5 |
Specification Test Results
Appearance: | Colorless or white to off- white crystalline powder |
Solution Test (1% in Methanol): | Clear and colorless |
Turbidity (1% in Methanol): | ≤ 4.0 NTU |
Water (KF): | ≤ 1.0 % |
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