Human AGRP - agouti-related ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemicals
KOA0862
76236-256EA 636 USD
76236-256
Human AGRP - agouti-related ELISA Kit, Rockland Immunochemicals
Assays ELISAs
Human AGRP - agouti-related AccuSignal ELISA Kit

  • Natural and recombinant human AGRP.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: sf21; Immunogen sequence: A21-T132

Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human AGRP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human AGRP standard solution and each sample be measured in duplicate.
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