Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS) ≥99%, white powder, Cell Culture Grade

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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76204-004EA 62.31 USD
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Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS) ≥99%, white powder, Cell Culture Grade
Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS)

Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.


  • Grade : Cell culture reagent
  • Purity :≥99%
  • Suitability : Suitable for cell culture
  • Key Applications : Used in electrophoresis buffer
  • Application Areas : Molecular Biology
  • Product Type : Biochemicals
  • Biochemical Category : Buffers
  • Extinction Coefficient : (E1%) EM (water): 259 nm = 10; 277 nm = 10 (Lit.)
  • Presentation : White Powder
  • pH : 9 - 10 (1% aq soln); Useful pH Range: 5.8-7.2 (Lit.)
  • pKa : 6.5 (25 °C)(Lit.)
  • Detection Method : FTIR
  • Solubility : Soluble in water (1 M - clear, colorless solution).
  • Sterilization of Solutions : For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving.

Formula: C₈H₁₉NO₅
Storage Temperature: Ambient
MDL Number: MFCD00002853
CAS Number: 6976-37-0
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Specification Test Results

Identity Test Passes
Purity Purity
FTIR Conforms to Standard C
Melting Point 101 - 106°C
pH (1% aq soln) 43353

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