TRKA Kinase Enzyme System, Promega

Supplier: Promega Corporation

V2931 V2932
PAV2931EA 631.42 USD
PAV2931 PAV2932
TRKA Kinase Enzyme System, Promega
Assays Enzyme Assays

Recombinant human TRKA (amino acids 440-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa membrane-spanning protein tyrosine kinase.


  • Easily Screen and Profile TRKA Kinase Inhibitors
  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity


Recombinant human TRKA (amino acids 440-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa membrane-spanning protein tyrosine kinase that is the functional receptor for nerve growth factor (NGF). NGF elicits the rapid phosphorylation of gp140trk on tyrosine residues leading to increased c-Fos expression, DNA synthesis and morphologic transformation. A decreased expression of TRKA on the striatal cholinergic neurons has been observed, which may contribute, when it reaches a crucial threshold, to the death of cholinergic neurons observed in Alzheimer disease. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: TRKA, 10ug (Human, recombinant; amino acids 440-end). MW: ~66kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT. TRKA NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4914/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.

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