MultiTox-Fluor Multiplex Cytotoxicity Assay, Promega

Supplier: Promega Corporation

G9200 G9201 G9202
PAG9200EA 327.37 USD
PAG9200 PAG9201 PAG9202
MultiTox-Fluor Multiplex Cytotoxicity Assay, Promega
Assays Cellular Assays

The MultiTox-Fluor Multiplex Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the number of live and dead cells simultaneously in culture wells.


  • A Fluorescent Assay That Measures Both Cell Viability and Cytotoxicity
  • Assay normalizes data for cell number
  • Reduces false-positive and false-negative results


The MultiTox-Fluor Multiplex Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the number of live and dead cells simultaneously in culture wells. The assay simultaneously measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. A second, cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 Substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity.

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