CytoTox-Fluor Cytotoxicity Assay, 10 ml, Promega

Supplier: Promega Corporation

G9260 G9261 G9262
PAG9260EA 179.89 USD
PAG9260 PAG9261 PAG9262
CytoTox-Fluor Cytotoxicity Assay, 10 ml, Promega
Assays Cellular Assays

The CytoTox-Fluor Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations.


  • A Fluorescent Assay to Measure the Relative Number of Dead Cells in Culture
  • Single-reagent-addition, homogeneous assay
  • Amenable to multiplexing with other luminescent cell-based assays


The CytoTox-Fluor Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations. The assay measures a distinct protease activity associated with cytotoxicity and uses a fluorogenic peptide substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110; bis-AAF-R110) to measure dead-cell activity, which has been released from cells that have lost membrane integrity. The bis-AAF-R110 substrate cannot cross the intact membrane of live cells and therefore gives no signal from live cells. The assay is designed to accommodate downstream multiplexing with several Promega luminescent assays or spectrally distinct fluorescent assay methods, such as assays to measure caspase activation, reporter gene expression or orthogonal measures of viability.

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