DMNPE-caged Luciferin
Supplier: OZ Biosciences
Synonyms:
D-Luciferin 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester
Packaged under nitrogen.
The cell permeable DMNPE-caged Luciferin is as a bioluminescent luciferase substrate used to measure intracellular functions since it readily crosses cell membranes. It is a D-luciferin ester analogue of luciferin.
- High purity
- Cell permeable: efficient luciferin delivery into intact cells even at neutral pH
- Ideal for in vivo applications
- Induces prolonged release of luciferin by the action of cells’ esterases
- Allows to follow changes in gene expression in live cells
- Suitable for in vitro experiments
Once the caged luciferin is inside the cells, active luciferin can be released by the action of endogenous intracellular esterases or by a flash of UV light. This implies:
1. A more efficient delivery of luciferin into intact living cells & improved sensitivity analysis of in vivo luciferase assays
2. A long-term measurement of luciferase activity since hydrolysis by esterases allows a continuous supply of active Luciferin
DMNPE-caged luciferin appears a good alternative to D-Luciferin classically used to measure luciferase activity in live cells. Indeed, the difficulty to deliver D-Luciferin into living cells renders in vivo quantification limited to a certain extent- for example, permeability issues at neurtral pH.
Shipped at room temperature.
Store at -20°C and protect from light.
Formula:
C₂₁H₁₉N₃O₇S₂ MW: 489.52 g/mol Storage Temperature: Freezer |
CAS Number:
223920-67-0 |
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