Anti-NCL Mouse Monoclonal Antibody (CF488A) [clone: 364-5 NCL/902]

Supplier: Biotium

BNC881205-100 BNC881205-500
75966-492EA 205.77 USD
75966-492 75966-494
Anti-NCL Mouse Monoclonal Antibody (CF488A) [clone: 364-5 NCL/902]
Antibodies
This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Type: Primary
Antigen: NCL
Clonality: Monoclonal
Clone: Clone 364-5 NCL/902
Conjugation: CF488A
Epitope:
Host: Mouse
Isotype: IgG's
Reactivity: Human
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