Baculovirus titering services to help customers maximize expression and reproducibility.
Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput, flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination for multiple conditions in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation period allowing for a single round of infection. Virus infected cells are then assessed by flow cytometric analysis for gp64 expression.
Ship 0.5 to 1 ml of virus stock (as little as 200 µl can be titered with advance notice).
Ship sterile aliquots in 2 ml screw cap tubes or equivalent. Ship overnight on cold packs.