ESF AF™ Insect Cell Culture Medium

Supplier: Expression Systems

99-300-01 99-300-20 99-300-50 99-300-10 99-300-01 99-300-10
101419-848EA 116.92 USD
101419-848 103740-078 103740-080 103740-082 77705-808 77705-810
ESF AF™ Insect Cell Culture Medium
Cell Culture Media

ESF AF™ insect cell culture medium is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells.


  • Animal component-free (100%)
  • Comparable to ESF 921 medium for robust cell growth and expression
  • Sf9 cell densities of 20×106 cells/ml or greater
  • Ideal for scale-up manufacturing
  • Works with a wide variety of baculovirus and host cell platforms
  • Optimized for applications such as production of sub-unit vaccines, virus-like particles and gene therapy vectors (rAAV - all serotypes)
  • Complete, ready-to-use, contains L-Glutamine
  • Adapted cells available in suspension


ESF AF is an animal origin-free formulation for insect cell culture. It has been thoroughly tested on Sf9, Sf21, Tni (High Five™) and S2 cell lines and is comparable to ESF 921 for growth and expression. ESF AF is the logical choice for further manufacturing under cGMP/FDA regulatory compliance.


Información para pedidos: Available in 1 L bottles, 10 L bags, 20 L bags and 50 L drums.

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