Fermentas Maxima™ Reverse Transcriptase, Thermo Scientific

EP0742 EP0743
101672-510EA 338.46 USD
101672-510 101672-512
Fermentas Maxima™ Reverse Transcriptase, Thermo Scientific
Nucleic Acid Reagents End-point PCR Enzymes and Kits

Maxima™ Reverse Transcriptase is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application.


  • Thermostable –90% active after incubation at 50 °C for 60 minutes in a reaction mixture
  • Active up to 60 °C
  • High yields of full-length cDNA as long as 20 kb
  • Efficient – complete cDNA synthesis in 15 to 30 minutes
  • Incorporates modified nucleotides
  • Developed through in vitro evolution


The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR


The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.


The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes.


Información de suministro: Supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25 °C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).

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