PIPES ≥99%, white powder for molecular biology

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: Piperazine-1,4-bis(2-ethanesulphonic acid), 2,2'-(Piperazine-1,4-diyl)diethanesulphonic acid, Piperazine-N,N'-bis-2-ethanesulphonic acid, 2,2'-(Piperazine-1,4-diyl)bis(ethanesulphonic) acid

0219483825 0219483880 0219483890
76177-364EA 41.41 USD
76177-364 76177-366 76177-368
PIPES ≥99%, white powder for molecular biology
PIPES

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.


  • Molecular Biology Reagent Grade
  • Presentation: White Powder
  • Effective buffering range: 6.1 - 7.5
  • For sterilization it is recommended to filter sterilize using a 0.2 µm filter.

Formula: C₈H₁₈N₂O₆S₂
MW: 302.37 g/mol
Melting Pt: > 300 °C (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00006159
CAS Number: 5625-37-6
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Specification Test Results

Identity Test Passes
Purity ≥99%
FTIR Conforms to Standard
UV/VIS: OD260 (0.1M in 0.2M NaOH) ≤0.05
UV/VIS: OD280 (0.1M in 0.2M NaOH) ≤0.05
Solubility (0.1M in 0.2N NaOH) Clear and Complete
pKa (25°C) 6.6-7.0
Moisture (LOD) ≤1.0%

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