Riboflavine (vitamin B2), orange powder USP
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
A vitamin/enzyme cofactor found in milk, eggs, malted barley, liver, kidney, heart, leafy vegetables. Richest natural source is yeast. Occurs in the free form only in the retina of the eye, in whey, and in urine; bioactive forms occurring in tissues and cells are riboflavin monophosphate and flavine-adenine dinucleotide.
Used as a photoinitiator for polymerization of polyacrylamide gels. It forms free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for electrophoresis. Riboflavin is commonly used in the stacking gel for non-denaturing polyacrylamide electrophoresis because native proteins can be senstive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerization until the gel is illuminated.
Riboflavin causes less denaturing than other initiators. Gel strength can be improved when used with PTO (Pyrithione). Soluble in 0.1 M Sodium Hydroxide (50 mg/ml - clear, yellowish-orange solution); only slightly soluble in water.
- USP Grade
- Presentation: Orange Powder
- Soluble in 0.1 M Sodium Hydroxide (50 mg/ml - clear, yellowish-orange solution); only slightly soluble in water.
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Formula:
C₁₇H₂₀N₄O₆ Melting Pt: 290 °C (dec., lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00005022 CAS Number: 83-88-5 |
Specification Test Results
| Identification | Passes |
| Assay | ≥98% |
| Specific Rotation (c=0.5, 0.05M NaOH) | -115° to -135° |
| Loss on Drying | ≤1.5% |
| Residue on Ignition | ≤0.3% |
| Limit of Lumiflavin | Passes |
| Organic Volatile Impurities | Meets Requirements |
| Particle Size | NLT 90% thru 80 mesh |
| Total Plate Count | NMT 10,000cfu/g |
| Yeast and Mold | NMT 1000cfu/g |
| E.coli | Negative |
| Salmonella | Negative |
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