iScript™ Reverse Transcription Supermix, Bio-Rad
iScript™ Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg – 1 pg total input RNA
- Amplify cDNA for genes with low expression levels
- Fast cDNA synthesis protocol
- 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
Storage temperature: Store at –20°C.
This single-tube supermix contains all the components for reverse transcription except RNA template. It is a sensitive, fast and convenient reagent for gene expression analysis.
Información de suministro: Kit includes iScript™ RT Supermix (gray cap, 25 or 100 reactions) and iScript™ No-RT Control Supermix (clear cap, 50 reactions).
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