Phenol Red Sucrose Broth, HiMedia Laboratories
Supplier: HiMedia
Phenol Red Sucrose Broth is used for sucrose fermentation studies of microorganisms.
Phenol Red Broth Medium is formulated and is recommended to determine the fermentation reaction of carbohydrates for the differentiation of micro organisms
Proteose peptone and beef extract serve as sources for carbon and nitrogen. Sodium chloride is the osmotic stabilizer. Phenol red is the pH indicator, which turns yellow at acidic pH i.e. on fermentation of sucrose. Gas formation is seen in Durhams tubes. All of the Enterobacteriaceae grow well in this medium. In addition to producing a pH colour shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odour from the culture medium.
This medium with various carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas.
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