ROS-Glo H2O2 Assay, Promega

Supplier: Promega Corporation

G8820 G8821
PAG8820EA 561.27 USD
PAG8820 PAG8821
ROS-Glo H2O2 Assay, Promega
Assays Enzyme Assays

The ROS-Glo H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions.


  • Specific, Direct Detection of Hydrogen Peroxide (H2O2)
  • Fast assay involves a simple two-reagent-addition protocol
  • Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme


The ROS-Glo H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatized luciferin substrate is incubated with sample and reacts directly with H2O2 to generate a luciferin precursor. Addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo Recombinant Luciferase to produce light signal that is proportional to the level of H2O2 present in the sample.


The addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H2O2 present in the sample.

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