Caspase Inhibitor Z-VAD-FMK
Supplier: Promega Corporation
Z-VAD-FMK is a cell-permeant pan caspase inhibitor.
- A Cell-Permeant Pan Caspase Inhibitor of Apoptosis
- Irreversibly binds to catalytic site of caspase proteases
- Provided in DMSO for convenient addition to cell culture or extracts
Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) is a cell-permeant pan caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and can inhibit induction of apoptosis. For inhibition of apoptosis, Z-VAD-FMK should be added at the same time that apoptosis is induced. Z-VAD-FMK is provided at 20mM in DMSO for convenient addition to cell culture or extracts. The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. The suggested concentration for use in the anti-Fas mAb-treated Jurkat cell culture model system is 20uM.
The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. The suggested concentration for use in the anti-Fas, mAb-treated Jurkat cell culture model system is 20 μm.
Z-VAD-FMK is provided at 20 mM in DMSO for convenient addition to cell culture or extracts.
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