Nitric Oxide Reagents and Kits, Biotium
Supplier: Biotium
Nitric oxide donors, nitric oxide synthase inhibitors, and nitric oxide detection reagents and kits.
- Nitric oxide donors available as stand-alone reagents and combination kit
- Nitric oxide synthase inhibitors available as stand-alone reagents and combination kit
- Nitric oxide detection kit based on Griess reagent
Nitric Oxide Donors: SIN-1 releases nitric oxide and forms superoxide spontaneously under physiological conditions and has been shown to be a potent vasodilator
Nitric Oxide Synthase Inhibitors: L-NMMA competitively inhibits nitric oxide synthase in a wide range of cells. L-NIL is a potent and selective inhibitor of nitric oxide synthase that exhibits about 28-fold greater selectivity for inducible nitric oxide synthase (IC50 = 3.3 µM) than for the rat brain constitutive enzyme (IC50 = 92 µM). L-NIO is a potent, irreversible inhibitor of neutrophil nitric oxide synthase. The NOS Inhibitor kit contains 7-NI, L-NMMA, L-NIL and L-NIO.
Nitric Oxide Detection: Griess reagent is used to detect nitrite photometrically. The reagent contains two chemicals, sulfanilic acid andN-(1-naphthalenediamine). Under acidic conditions sulfanilic acid is converted by nitrite to a diazonium salt, which readily couples with N-(1-naphthalenediamine) to form a highly colored azo dye that can be detected at 548 nm. Under physiological conditionss, NO is unstable and is rapidly oxidized to a mixture of nitrite and nitrate. In order to measure NO levels indirectly from nitrite, nitrate must be reduced to nitrite enzymatically using nitrate reductase (not included) before performing the assay so that the total amount of nitrite can be measured. The kit includes ready-to-use Griess reagent, nitrite standard solution, and a detailed protocol.
SNAP releases nitric oxide under physiological conditions and also been shown to be a potent vasodilator. Similar to SNAP, S-nitrosogluthathione releases nitric oxide spontaneously under physiological conditions. Spermine NONOate releases nitric oxide spontaneously under physiological conditions, and is useful for reliable NO generation in solution. DEA-NONOate releases nitric oxide spontaneously under physiological conditions. Useful for reliable NO generation in solution. The Nitric Oxide Generation Kit contains SIN-1, SNAP, S-Nitrosoglutathione, spermine NONOate, and DEA-NONOate.
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