Protease Substrates, Biotium

Supplier: Biotium

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89138-354EA 178.66 USD
89138-354 89138-356 89138-358 89138-360 89138-362 89138-364 89138-366 89138-368 89138-370 89138-372 89139-508
Protease Substrates, Biotium
Assays Enzyme Assays

Fluorogenic substrate for caspase 3, cathepsins, plasmins, granzyme B, and more.


  • Rhodamine 110 for use as standard for rhodamine 110-based peptidase substrates
  • AMC and Rhodamine 110-based protease substrates


Rhodamine 110 and R110-PEG can be used as a reference standard for rhodamine 110-based peptidase substrates.


Z-FR-AMC is a substrate for serine proteases, including cathepsins, kallikrein and plasmin.


Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.


BZAR is a substrate for serine proteases and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.


Ac-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
Z-FR-R110 is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.
D2R is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
Z-AAD-R110 is a substrate for granzyme B. Granzyme B from T lymphocytes and natural killer cells induces apoptosis in target cells by activating caspases and causing mitochondrial cytochrome C release.
Z-AA-R110-PEG is a substrate for elastate.

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