Nutrient Mixture F-12 Ham Mammalian Cell Culture Medium, HiMedia

Supplier: HiMedia

AT025-5L AT144-20L AT144-5L AT025-50L AT025-20L
95016-620EA 64.57 USD
95016-620 89138-010 89138-012 95016-618 89138-008 95057-920 95062-756 95062-758 89138-006 95016-622
Nutrient Mixture F-12 Ham Mammalian Cell Culture Medium, HiMedia
Cell Culture Media

Ham’s Nutrient mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells.


  • Formulated for use with or without serum
  • Designed for serial propagation and cloning of two CHO cell lines
  • Medium of choice for the growth of cells of rodent origin
  • Also for cloning of myeloma and hybridoma cells


Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham’s nutrient mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells.

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