OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence), Cell Biolabs
Supplier: Cell Biolabs
The OxiSelect™ In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types
This assay kit measures total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anions. It is suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants. The detection sensitivity limit is 10 pM for DCF and 40 nM for hydrogen peroxide.
This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
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