Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions. Stored in a cool, dry, and well-ventilated area, protected from direct sunlight.
- Popular anionic, bile-acid detergent for many laboratory uses
- Effective in disrupting and dissociating many types of protein interaction
- Can be removed from solution by dialysis
- Useful for elution or regeneration of certain kinds of affinity columns
- High-purity compound with low UV absorptivity
Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods
The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW
2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.
Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.
Precaución: For Research Use Only. Not for use in diagnostic procedures.